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Western blot analysis of expression of MLL fusion genes and deletion mutants in U937T cells. Cells were induced for 48 hr in tetracycline-free medium. Cell extracts were prepared, run on 6% (MLLΔT, MLL-AF9; A) or 12% (MLL2AT, MLL3AT; B) polyacrylamide/Tris–glycine gels, and blotted to nitrocellulose, and proteins were detected with M2 anti-FLAG monoclonal antibody.
