Figure 2.

Representative Western immunoblots of phosphorylated ERK1,2 and total ERK1,2 proteins from lysates of human HCC cell lines following treatment with PD098059, U0126, and PD184161. Actively dividing cells (Hep3B, HepG2, PLC, and SKHep) were treated for 1 hour with one of the following mitogen-activated protein kinase kinase (MEK) inhibitors: PD098059, U0126, or PD184161 (0.1 and 1.0 µM). Following treatment, the cells were lysed, and Western immunoblots were performed using antibodies to phosphorylated ERK1,2 (A) and total ERK1,2 (B). Similar results were obtained in at least two independent experiments.