Figure 7.

The effect of single and multiple oral dosing with the MEK inhibitor PD184161 on the expression of phosphorylated ERK1,2 (A) and total ERK1,2 (B) in nude mouse Hep3B tumor xenografts and lung. Homogenates from Hep3B tumors were prepared from treated and control mice and assessed by Western blot analysis for phosphorylated ERK1,2 and total ERK1,2 levels. Representative Western blot analyses are shown here. Results were reproducible in multiple assays. Densitometric analysis was performed, and results were analyzed by t-test (P < .05).