Figure 1.

The effect of hyperglycemia and STZ on O-GlcNAc accumulation in pancreatic islets in vivo. Fasted rats were infused with glucose either without STZ or 15 min after the STZ-pretreatment, and the pancreases were examined for the distribution of RL2-detectable glycoprotein. (A and D) Overnight fasted. (B and E) 45 min after initiation of glucose infusion (30 min with [glucose]>20 mM. (C and F) The glucose infusion was terminated after 45 min and replaced with an insulin infusion that was continued for a further 135 min. The blood glucose was <10 mM for about 1 hr when the pancreases were examined. (D–F) STZ pretreatment. (Magnification: frames 1, ×200; frames 2, ×25.).