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. 2006 Jun;8(6):499–509. doi: 10.1593/neo.05847

Figure 2.

Figure 2

Representative RT-PCR from microdissected tubules and seminoma cells using primer pairs for Cx43 (A) and β-actin (B). Seminiferous tubules with normal spermatogenesis (lane 2) and seminiferous tubules with both residual spermatogenesis and CIS (lane 3) revealed a 137-bp PCR product for Cx43. Tubules resembling only CIS and Sertoli cells (CIS-only tubules) were found positive or in most cases negative (lanes 4 and 5). Seminoma cells isolated from different seminomatous stages (pT1–pT3) showed no signal for Cx43 mRNA (lane 6). Lane 1: 100-bp DNA ladder.