Figure 1.
Identification of an interaction between ERβ and MAD2 in the yeast two-hybrid system. (Upper) ERβ fused to the Gal4 BD (mERβ-BD) interacts with clone EC-1 (EC-1-AD) isolated from the ovine pulmonary artery endothelial cell yeast two-hybrid system library. ERβ-BD and the Gal AD vector alone fail to interact, as do the BD vector alone and EC-1-AD. The positive interaction of mERβ-BD with EC-1-AD occurs similarly in the presence and the absence of estrogen (data not shown). (Lower) ERα-BD failed to interact with EC-1-AD in the two-hybrid system in the presence of 10−8 M estradiol under conditions in which ERα-BD interacted with both of the known nuclear hormone receptor coactivator proteins TIF2 and TRIP1.