Protein–protein interaction studies with ERβ, ERα, and MAD2.
(A) Protein–protein interaction studies with
[S35]mERβ and GST-EC-1. [S35]mERβ was
incubated with GST beads alone or GST-EC-1 beads followed by SDS/PAGE
and autoradiography. The first lane shows 10% of
[S35]mERβ input. (B) Protein–protein
interaction studies with [S35]hERα and ERβ or EC-1.
[S35]hERα was incubated with GST-mERβ (positive
control), GST beads alone, or GST-EC-1, followed by SDS/PAGE and
autoradiography. The first lane shows 7% of [S35]hERα
input.