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. 2000 Mar 7;97(6):2836–2839. doi: 10.1073/pnas.050580997

Figure 4.

Figure 4

Protein–protein interaction studies with full-length or truncated ERβ and MAD2. (A) Protein–protein interaction studies with [S35]hMAD2 and mERβ. [S35]MAD2 was incubated with GST beads alone or GST-mERβ beads followed by SDS/PAGE and autoradiography. The first shows 15% of [S35]MAD2 input. (B) Protein–protein interaction studies with [S35]hMAD2 and full-length ERβ or several ERβ truncation mutants. [S35]MAD2 was incubated with GST beads alone, GST-full-length ERβ (GST-rERβ), GST-mERβ173–485, GST-mERβ1–208 beads, or GST-mERβ 173–208, followed by SDS/PAGE and autoradiography. The first lane shows 7% of [S35]MAD2 input.