Table 1.
MAD2 interactions with ERβ and ERα in the yeast two-hybrid system
| BD | AD | LacZ assay |
|---|---|---|
| mERβ | EC-1 (MAD2 7-205) | + |
| mERβ | MAD2 45-205 | − |
| mERβ 1-208 | EC-1 | + |
| mERβ 1-172 | EC-1 | − |
| mERβ 173-208 | EC-1 | + |
| BD alone | EC-1 | − |
| EC-1 | mERβ | + |
| EC-1 | mERβ 173-485 | + |
| EC-1 | mERβ 209-485 | − |
| EC-1 | — | − |
| EC-1 | AD alone | − |
| mERβ | MAD2 7-160 | + |
| mERβ 1-208 | MAD2 7-160 | − |
| mERβ 173-208 | MAD2 7-160 | − |
| hERα | EC-1 | − |
| hERα | mERβ | + |
| hERα | TIF2 | + |
| hERα | TRIP1 | + |
Summary of histidine and β-galactosidase reporter activation for various ERβ and ERα constructs tested for their interaction in the yeast two-hybrid system with the MAD2 clone EC-1 (corresponding to amino acids 7-205 of hMAD2) and MAD2 mutants.