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Effect of CD38 and MHC class II modulation on the Sag-induced T cell proliferation. Whole purified T cells (4 × 104 cells/well) were cultured with SEA (1 ng/ml) in the presence of irradiated monocytes (8 × 103 cells/well) pretreated or not by CD38 or MHC class II mAbs as described in Fig. 9. [3H]thymidine incorporation measured at day 4 for T cells in the presence of untreated irradiated monocytes was 54.410 ± 5.275 cpm.
