Figure 5.
An intracellular calcium flux is mediated via CD38 on monocytes. Purified monocytes were loaded with indo-1, washed, and tested for calcium mobilization. After measurement of basal level, mAbs of the following specificity were added (first arrow): (A) CD38, (B) CD3, (C) anti-MHC Class II, (D) CD38 + anti-MHC Class II. After 5 min, these were crosslinked by addition of a rabbit anti-mouse F(ab′)2 fragment at a predetermined concentration known not to mobilize intracellular calcium (second arrow).