Figure 8.
CD38 ligation on human monocytes induces tyrosine phosphorylation of the fgr and hck tyrosine kinases. After incubation with either no mAb, CD38 mAb, F(ab′)2 IB4, or anti-MHC Class II mAb, purified monocytes were lysed and immunoprecipitates prepared by using either an anti-hck Ab (A and B) or an anti-fgr Ab (C and D). After blotting, filters were probed with an antiphosphotyrosine mAb (A and C). The filter shown in A was stripped and reprobed with an anti-hck Ab revealing identical amounts of hck in all samples (B). The filter shown in C was stripped and reprobed with an anti-fgr Ab revealing identical amounts of fgr in all samples (D).