Figure 4.

Effects of immunoneutralization of IFNγ on a stress-induced enhancement of skin DTH in wild-type mice. Animals were injected with rat IgG (A) or anti-IFNγ (B). Then, 1.5 h after antibody administration, control animals were left undisturbed, while stressed animals were restrained for 2.5 h. The right pinnae of all animals were then challenged with DNFB (0.2%). A time course of changes in the thickness of challenged pinnae is shown. Vehicle-injected wild-type mice showed a significant stress-induced enhancement of skin DTH (n = 5; *, P < 0.05, independent t test). In contrast, wild-type mice injected with anti-IFNγ antibody failed to show this enhancement (n = 3).