Figure 1.

Generation of recombinant adenoviral vectors. Depicted are the recombinant adenovirus type 5 (Ad5) genome. Ad5-Lps(n)-HA and Ad5-Lps(d)-HA are the vectors containing the Lps(d)/Ran cDNA and Lps(d)/Ran cDNAs tagged with an HA-epitoped in-frame with the codon. Ad5-GFP and Ad5-LacZ are vectors containing the GFP and lacZ genes. Expression cassettes replacing the E1 region include the cytomegalovirus promoter, the inserted cDNAs with their own polyadenylation signals, flanked by 5′ inverted terminal repeat (ITR), and encapsidation signal (ψ).