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Adenoviral titer determination. Cytopathic effect assay was performed as described in Materials and Methods. pfu, plaque-forming unit. (Lower) PCR results on GP/E fibroblasts with or without infection with various dilutions of either Ad5-Lpsn/Ran (n) or Ad5-Lpsd/Ran (d) virus. MOI is a ratio of 1 cell/number of infectious virus particles. The 127-bp band shows the molecular weight visible upon brighter exposure.
