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. 2000 Mar 7;97(6):2858–2863. doi: 10.1073/pnas.050587897

Figure 2.

Figure 2

Interaction of M1+ and M1− streptococci with A549 (A) and human tonsillar epithelial (HTE) (B) cells. S. pyogenes 90-226 (M1+) and 90-226 emm1∷Km (M1−) were suspended in RPMI 1640 medium or medium supplemented with 10% FBS, just before infection of monolayers. Hatched bars, percent of bacterial cfu that adhered to host cells; open bars, percent of bacteria internalized in the presence of 10% FBS; solid bars, percent of bacteria internalized in the absence of FBS. Some bars are too short to be visible in the figure. Data are the means ± SEM of two (A) or three (B) experiments in which each assay was performed in triplicate. HTE cell experiments were performed with cells obtained from three different donors.