Table 3.
Effect of SJ755 on the formation of α5β1-Fn-M1 protein complexes in vitro
| Solid phase | First
incubation
|
Second incubation
|
Absorbance at 405 nm | |||
|---|---|---|---|---|---|---|
| Fn | SJ755 | Anti-α5β1 | M1 | SJ755 | ||
| No protein | + | − | − | + | − | 0 |
| Ovalbumin | + | − | − | + | − | 0 |
| Integrin α5β1 | + | − | − | + | − | 0.49 ± 0.10 |
| " | − | − | − | + | − | 0.03 ± 0.01 |
| " | + | − | − | − | − | 0.02 ± 0.01 |
| " | + | + | − | + | − | 0.04 ± 0.01 |
| " | + | − | + | + | 0.31 ± 0.08 | |
| " | + | − | + | + | − | 0.04 ± 0.01 |
Assays to detect binding of M1 protein to α5β1 or α5β1-Fn complexes were performed as described in Materials and Methods. Data are the means ± SEM from two independent experiments in which each assay was performed in triplicate. Plus and minus signs indicate the presence or absence of a given reactant in an incubation step.