Table 2.
Comparison of factors affecting reproductive fitness in selected lines of the Black, Red, and Green groups of Anopheles gambiae Keele (C, unselected control; R, refractory; and S, susceptible). Treatments that resulted in significant differences between lines are denoted by different letters. n = 45–80. Mean number of mosquitoes taking a bloodmeal (a, b) and the percentage of mosquitoes dying before oviposition (e, f, g) were compared using chi-squared analysis; df = 2, differences significant at P < 0.05. Hematin production was used as a measure of bloodmeal size and data analyzed by one-way analysis of variance and Tukey's test following an Anderson-Darling test for normality (x, y, P < 0.05). Analyses were performed on mosquitoes from the 12th and 13th generations.
| Uninfected blood |
Infected blood feed |
Infected blood feed/stress |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| C | R | S | C | R | S | C | R | S | |
| Black group | |||||||||
| Taking a bloodmeal (%) | 75.0 | 70.0 | 83.0 | 98.3 | 83.3 | 96.7 | 92.8 | 75.7 | 89.2 |
| Dying before oviposition (%) | 3.0 | 8.0 | 6.5 | 8.8 | 10.0 | 5.0 | 4.0e | 31.0f | 6.3g |
| Hematin production (μg) | 8.1 | 8.3 | 8.0 | 12.3x | 13.2xy | 14.9y | 10.4 | 9.3 | 10.2 |
| Red group | |||||||||
| Taking a bloodmeal (%) | 100.0 | 74.0 | 87.0 | 98.0 | 89.0 | 97.0 | 56.0a | 73.0b | 85.0b |
| Dying before oviposition (%) | 5.0 | 10.0 | 3.7 | 6.8 | 5.0 | 10.0 | 33.3e | 15.5f | 5.9g |
| Hematin production (μg) | 10.0x | 7.9y | 9.7xy | 11.0x | 16.6y | 10.5x | 3.12x | 5.65y | 4.4x |
| Green group | |||||||||
| Taking a bloodmeal (%) | 85.2 | 89.2 | 80.0 | 78.0 | 70.0 | 81.0 | 87.0 | 91.0 | 92.0 |
| Dying before oviposition (%) | 8.6 | 4.5 | 3.0 | 21.9 | 30.5 | 19.4 | 12.7 | 9.1 | 7.9 |
| Hematin production (μg) | 7.1x | 7.8xy | 8.2y | 11.0 | 11.3 | 10.2 | 12.7x | 14.0xy | 15.6y |