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. Author manuscript; available in PMC: 2006 Oct 13.
Published in final edited form as: Arthritis Rheum. 1998 Aug;41(8):1398–1406. doi: 10.1002/1529-0131(199808)41:8<1398::AID-ART8>3.0.CO;2-B

Figure 1.

Figure 1

Interleukin-4 (IL-4) inhibits IL-1 induction of collagenase (Coll) and stromelysin (Stro) mRNA in human synovial fibroblasts (HSF). Total RNA was isolated at the indicated time points after addition of 100 ng of IL-1/ml alone or in combination with 10 ng of IL-4/ml to cultures of human synovial fibroblasts. A, Northern blots were hybridized to cDNA probes corresponding to collagenase (matrix metalloproteinase 1 [MMP-1]), stromelysin 1 (MMP-3), and GAPDH. B, Blots were quantitated by scanning densitometry and normalized to levels of GAPDH. Shown are data from 3 independent experiments with RNA isolated from HSF.