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. Author manuscript; available in PMC: 2006 Oct 13.
Published in final edited form as: Arthritis Rheum. 1998 Aug;41(8):1398–1406. doi: 10.1002/1529-0131(199808)41:8<1398::AID-ART8>3.0.CO;2-B

Figure 9.

Figure 9

Binding of transcription factor activator protein 1(AP-1) is not affected by interleukin-4 (IL-4). Nuclear extracts were isolated from human synovial fibroblast cultures treated for 1hour with 100 ng of IL-1β/ml, 10 ng IL-4/m1, or both IL-1 and IL-4, as well as from control cultures. Binding of 5 μg of nuclear extract to a 32P-labeled oligo(dT) probe corresponding to the AP-1 site in the stromelysin promoter was determined by electrophoretic mobility shift assay. P = probe alone, without nuclear extract, C = control, without interleukin.