TABLE 3.
Formation of retinal cells in crsp150hi2143 mutant embryos and wild-type siblings (78 hpf)
Assay | wt ± SD | Mutant ± SD | P-value |
---|---|---|---|
Thickness of amacrine layer | 38.0 ± 2.9 μm (n = 4) | 34.7 ± 2.5 μm (n = 3) | P = 0.18 |
rhodopsin-stained volume | 209,400 ± 71,627 (n = 8) | 175,621 ± 40,695 (n = 8) | P = 0.27 |
rhodopsin staining intensity (average per pixel) | 192 ± 11 (n = 8) | 192 ± 14 (n = 8) | P = 0.96 |
Nuclear TOTO3 staining intensity (average per pixel) | 110 ± 14 (n = 8) | 111 ± 16 (n = 8) | P = 0.89 |