TABLE 2.
Plasmids used in this study
| Plasmid | Relevant genotypea | Source |
|---|---|---|
| pWJ659 | CEN LEU2 TOP3 | Gift of J. Weinstein |
| pWJ171 | CEN LEU2 TOP3 | Wallis et al. (1989) |
| pRS415 | CEN LEU2 | Sikorski and Hieter (1989) |
| pWJ1092 | CEN URA3 TOP3 | This study |
| pWJ1114 | URA3 TOP3 | This study |
| YCp50 | CEN URA3 TOP3 | Rose et al. (1987) |
| pRS306 | URA3 | Sikorski and Hieter (1989) |
| pWJ1130 | CEN URA3 top3–E447K, S583L | This study |
| pWJ1203 | URA3 top3–E447K, S583L | This study |
| pRS304 | TRP1 | Sikorski and Hieter (1989) |
| pWJ1174 | TRP1 iYLR237 | This study |
| pRS425 | 2μ LEU2 | Sikorski and Hieter (1989) |
| pRS1372 | 2μ LEU2 top3–E447K, S583L | This study |
| pWJ1047 | CEN URA3 Gal-A-HpaI-B | Gift of R. Reid |
| pWJ1067 | CEN URA3 Gal-TOP3 | This study |
| pWJ1074 | CEN URA3 Gal-top3–Y356F | This study |
| pWJ1085 | CEN LEU2 top3–Y356F | This study |
| pWJ1113 | URA3 top3–Y356F | This study |
| pRS417 | CEN ADE2 | Brachmann et al. (1998) |
| pWJ1189 | CEN URA3 ADE2 TOP3 | This study |
| pWJ1279 | CEN URA3 Gal-PIF1 | This study |
| pWJ1280 | CEN URA3 Gal-pif1–K264R | This study |
| pWJ1281 | CEN URA3 Gal-RRM3 | This study |
| pWJ1345 | CEN HIS3 Gal-A-HpaI-B | This study |
| pWJ1346 | CEN HIS3 Gal-TOP3 | This study |
| pWJ1347 | CEN HIS3 Gal-top3–Y356F | This study |
| pUH7 | ura3∷HIS3 | Cross (1997) |
| pRS413 | CEN HIS3 | Sikorski and Hieter (1989) |
| pWJ1315 | CEN HIS3 NUP49 | This study |
| pWJ1323 | CEN HIS3 CFP-NUP49 | This study |
| pRS416 | CEN URA3 | Sikorski and Hieter (1989) |
| pWJ1348 | CEN URA3 CFP-NUP49 | This study |
| pLH7 | leu2∷HIS3 | Cross (1997) |
| pCox4-DsRed.T4 | CEN LEU2 COX4–DsRed | Bevis and Glick (2002) |
| pWJ1326 | CEN HIS3 COX4–DsRed | This study |
| pWJ1249 | CEN TRP1 C-HpaI-D | Gift of R. Reid |
| pWJ1277 | CEN HIS3 NOP1 | This study |
| pWJ1299 | CEN HIS3 NOP1–CFP | This study |
| pWJ1327 | CEN URA3 NOP1–CFP | This study |
| pWJ1321 | CEN HIS3 NOP1–DsRed | This study |
| pWJ1322 | CEN URA3 NOP1–DsRed | This study |
| YEp51B | 2μ LEU2 | Akada et al. (1997) |
| Clone 1 | 2μ LEU2 PIF1 | Akada et al. (1997) |
| Clone 2 | 2μ LEU2 PIF1 | Akada et al. (1997) |
| HCS2 | 2μ LEU2 TOP3 | Akada et al. (1997) |
| pWJ1246 | Clone 1 + Δpif1 | This study |
| pWJ1286 | Clone 1 + pif1–K264R | This study |
| pL1580 | CUP1∷URA3-SUP11-o | Keil and McWilliams (1993) |
a
Plasmid sequences and/or maps are available upon request.