TABLE 3.
High-copy PIF1 suppresses the top3Δ cell-cycle defect
| % of cells in each cell-cycle stagea
|
||||
|---|---|---|---|---|
| G1 | S | G2/M | ||
| Wild type | + p2μ | 54 | 23 | 23 |
| + p2μ-PIF1 | 52 | 24 | 24 | |
| + p2μ-pif1-KR | 53 | 23 | 24 | |
| top3Δ | + p2μ | 34 | 17 | 49 |
| + p2μ-PIF1 | 50 | 20 | 30 | |
| + p2μ-pif1-KR | 50 | 21 | 29 | |
a
As described in materials and methods, cell-cycle stage classification was determined via microscopy on the basis of both cellular morphology and nuclear position. Over 750 cells from logarithmic cultures grown in SC-Leu were viewed for each strain + plasmid background. Strains used are W1588-4C (MATa TOP3) and a W1958 haploid segregant (MATa top3∷TRP1). Plasmids used are the LEU2-marked YEp51B (p2μ), clone 1 (p2μ-PIF1), and pWJ1286 (p2μ-pif1-KR).