TABLE 2.
Putatively functional sequences and pseudogenes obtained by cloning the PCR product from three different protocols: standard PCR, high-stringency PCR (with DMSO), and (RT–PCR)
| Putatively functional
|
Pseudogenes: | ||
|---|---|---|---|
| Group 1 | Group 2 | Groups 3–8 | |
| Standard PCR | 3 | 9 | 23 |
| PCR with DMSO | 18 | 1 | 0 |
| RT–PCR | 5 | 4 | 7 |
The eight groups represent well-supported clades in a maximum-likelihood tree, of which two are putatively functional (groups 1 and 2) and the rest are pseudogenes (Figure 2).