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(A) The [psi−] wild-type (TKY603), parental eEF1Bα-deficient (TKY604), or SBD5-11 (TKY781-787) strains were grown in YEPD and diluted to an A600 of 1.0. Ten-fold serial dilutions were spotted and grown at 13°, 24°, 30,° and 37° for 2–8 days. (B) Equal amounts of total yeast proteins from strains, as in A, as determined by Bio-Rad assay, were analyzed by SDS–PAGE and Western blot analysis with polyclonal antibodies to yeast eEF1A and yeast Rpa1p (as a loading control).
