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Wild type (TKY631, pTEF5 URA3, eEF1Bα-deficient (TKY767) with pRS316 and TKY767 expressing the indicated TEF2 SBD double-mutant plasmid strains were grown in C-Ura and diluted to an A600 of 1.0. Ten-fold serial dilutions were spotted onto C-Ura plates and grown at 24°, 30°, and 37° for 2–8 days.
