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A wild-type strain (MC213 pTEF1 TRP1) and strains TKY961–965 obtained by transforming the tef5∷TRP1 fragment into TKY789, 847–849, and 852 and selected by growth on C-Trp were grown in YEPD and diluted to an A600 of 1.0. Ten-fold serial dilutions were spotted onto YEPD plates and grown at 13°, 24°, 30°, and 37° for 2–8 days.
