TABLE 5.
Total translation and nonsense and missense effects of the SBD mutant strains in the presence and the absence of eEF1Bα
| Total translation relative to wild type (% change) | Nonsense suppression (UAA) (Rtest/Rcon)a | Missense suppression (Rtest/Rcon)a | |
|---|---|---|---|
| Wild type | 100 | 0.029 ± 0.01 | 0.01 ± 0.004 |
| R164K + eEF1Bα | 100 | ND | ND |
| R164K − eEF1Bα | 125 | 0.034 ± 0.01 | 0.012 ± 0.004 |
| A117V + eEF1Bα | 100 | ND | ND |
| A117V − eEF1Bα | 75 | 0.028 ± 0.01 | 0.008 ± 0.003 |
| T22S + eEF1Bα | 65 | ND | ND |
| T22S − eEF1Bα | 65 | 0.073 ± 0.02 | 0.01 ± 0.004 |
| A112T + eEF1Bα | 75 | ND | ND |
| A112T − eEF1Bα | 75 | 0.079 ± 0.02 | 0.012 ± 0.003 |
| D156N + eEF1Bα | 125 | ND | ND |
| D156N − eEF1Bα | 100 | ND | ND |
ND, not determined.
a
Rtest/Rcon: the activity ratio derived from lysates expressing test cassettes divided by the activity ratio of the control reporter. Standard deviations were calculated using a minimum of four samples.