TABLE 1.

Breakpoints of spontaneous mrt-2 and clk-2(mn159) deletion products

lon-2(e2755) (mrt-2)

AAACATAAATCTTTTTGTCATGCCATTTTTGGAAA..7073..gtcgtaaatttcaattacacacgcggcttcttttt

AT (insert)

caggtgagttaaaaatatttgggaggggggggggg..6623..ATCATAGAAAAACGTTTTTGTAATTTTTTTAAATT

(polypurine tract)

unc-17(e2754) (mrt-2)

TTTAAAATTTAAAAAAAGGTCTGAACTATTTTTTT..24834..ggatttttttttggattttttacaacggaaaaatt

cgcggggagtggggggggggggggggggggggggg..26647..GGATTTGCTACCGGTTGTCGAAAAAGGAGTTCAAA

(polypurine tract)

unc-60(e2763) (mrt-2)

AAAAATTTCCATGTATTTCCACATTCCACTTCCCA..11548..aactcctattttcagctgcccagacaacgccccag

AAgtacaggcctccgagatgtcggacctcgacgag..11690..AAGAGCGTGAAGAGCGACCTGATGTCCAACCAAAG

(hairpin)

vab-8(e2764) (mrt-2)

CACACCGTTTTCTTCTCGAGACCACCCATAAAAGT..26399..cgtgggtggcgcaaccagaaatactgtcggcccgt

(hairpin)

tcaatcaaatactgatatggatgtttctgaaatgg..29834..GTTTACCGGAGAGCAAGCCATTTGGAAGAATATGC

(hairpin)

dpy-10(e2760) (clk-2 mn159)

TTAATGAAAAATTAAAACCGCTAGAGCTTGAAAAT..4183..ccaacaaacgcagcttcttgagaaaaattaattgg

CTAAACAAATAAACAATAAAAAAACTAAAAAATAAAAAAAAAAA (insert)

acaatgtagatgagaagagaagtactacggtaggt..7799..ATGAGTGAGTGTGTGGATCGAAAAGTGAAATTTCAAAGTGAGC GGGGGGCATAGGGTGGTGGCGGCGCCCGCCGCGCGCGC

(direct repeat and G/C-rich tract)

Sequences present at the breakpoints are in uppercase. Deleted sequences are in lowercase. Positions of breakpoints relative to the cosmid sequence for each gene are indicated (http://www.wormbase.org). Microhomology at breakpoints is underlined and in italics. Novel DNA sequence inserted between each breakpoint during NHEJ is indicated. DNA sequences with unusual qualities are underlined, in boldface, and defined in parentheses beneath the sequence. A direct repeat at the e2760 breakpoint is italicized, underlined, and in boldface.