TABLE 3.
unc-58(e665) background | Trial | Plates with revertants/ total plates | f(0) | Mutation frequency (revertants/gamete) | Spontaneous mutations/gamete |
---|---|---|---|---|---|
Wild type | 1 | 2/40 | 0.95 | 5.52 × 10−6 | |
2 | 0/40 | 1 | 0 | ||
3 | 0/40 | 1 | 0 | ||
4 | 0/40 | 1 | 0 | ||
5 | 1/40 | 0.975 | 2.73 × 10−6 | ||
6 | 0/40 | 1 | 2.73 × 10−6 | ||
7 | 1/40 | 0.975 | 0 | ||
8 | 0/40 | 1 | 0 | 1.4 ± 1.9 × 10−6 | |
cep-1(lg12501) | 1 | 1/40 | 0.975 | 2.73 × 10−6 | |
2 | 0/40 | 1 | 0 | ||
3 | 1/40 | 0.975 | 2.73 × 10−6 | ||
4 | 0/40 | 1 | 0 | ||
5 | 0/40 | 1 | 0 | ||
6 | 0/40 | 1 | 0 | ||
7 | 0/40 | 1 | 0 | ||
8 | 0/40 | 1 | 0 | 0.68 ± 1.2 × 10−6 | |
cep-1(gk138) | 1 | 2/40 | 0.95 | 5.52 × 10−6 | |
2 | 0/40 | 1 | 0 | ||
3 | 1/40 | 0.975 | 2.73 × 10−6 | ||
4 | 2/40 | 0.95 | 5.52 × 10−6 | ||
5 | 0/40 | 1 | 0 | ||
6 | 0/40 | 1 | 0 | 2.3 ± 2.5 × 10−6 |
Calculations were made as for Table 2, except that 60-mm plates were used, yielding an estimated 9320 haploid genomes sampled per plate.