TABLE 1.

Oligonucleotide sequences

Oligo
atpA forward	5′-GTC TGG TCC GAT CGT TTA GC-3′
atpA reverse	5′-TAA AGA GGG CGA TCT TGT CA-3′
cox1 forward	5′-TGG GCA CAT GCT TCT CAG TA-3′
cox1 reverse	5′-CTC TTC AAC AGC CCA AGG AC-3′
Q forward	5′-ACG TCG CCT GGG AAA GC-3′
Q reverse	5′-TCC GCG ATA ATG GAA TGC A-3′
Probe X	5′-6-FAM-CGG CCT GGT GGT CGG CGT AAC-BHQ1-3′
Probe A	5′-6-FAM-TGA CAT TTG TCG ATA TGC CAC CG-BHQ1-3′
Probe D	5′-HEX-AGA CAT TTG CCG GTA TGC CAC C-BHQ1-3′

Primers and probes for PCR and quantitative PCR of mitochondrial atpA and cox1 that are specific to Silene vulgaris are shown. Sequences underlined denote cut sites for restriction enzymes TaqαI, which cuts allele A, and MspI, which cuts allele D. Bases shown in italics are allele specific. Probe sequences also include fluorophores with which they are labeled (i.e., 6-FAM and HEX).