Figure 2.

Inhibition of HCMV pro-gB processing results in mislocalization of the envelope precursor. (A–D). U-373 cells grown on coverslips were infected with HCMV Towne (moi = 0.2) and cultured in the absence (A and C) or presence (B and D) of 8 μM α₁-PDX. At 5 days after infection, cells were fixed, permeabilized, and processed for immunofluorescence microscopy. HCMV gB molecules (A and B) were detected with mAb 27-156, and HCMV gH molecules (C and D) were detected with mAb 14-4B. (E and F) U-373 MG cells grown on coverslips were infected with a recombinant adenovirus expressing HCMV gB (moi = 10) and incubated for 48 h in the absence (E) or presence (F) of 8 μM α₁-PDX. Cells were fixed and permeabilized, and epitope-tagged gB molecules were localized by immunofluorescence microscopy with an anti-FLAG mAb.