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. Author manuscript; available in PMC: 2006 Oct 13.
Published in final edited form as: Neuroscience. 2006 Mar 6;139(4):1435–1448. doi: 10.1016/j.neuroscience.2006.01.020

Fig. 3.

Fig. 3

fabp7 and chordc1 are differentially expressed at ZT6 and ZT18 in specific hypothalamic brain regions. Northern blotting on RNA samples independent from those used for microarray analysis revealed differences in expression levels for (A) fabp7 and (B) chordc1 mRNA at ZT6 and ZT18 in VLPO, SCN and LH. Densitometry was used to compare the expression ratio in each case at ZT6 compared with ZT18 (reported as fold change) with gapdh. The expression of fabp7 mRNA at ZT6 was significantly higher and chordc1 was significantly lower than at ZT18. Each value represents the average±S.E.M. Each lane represents RNA pooled from 12 individual animals. SCN, LH, N=3; VLPO, N=2. * P<0.05, ** P<0.01 (t-test). Light shaded bar represents fold change of fabp7 (A) and chordc1 (B). Dark shaded bar represents fold change in gapdh in (A) and (B). Size of mRNA bands was chordc1, 2200 bp; fabp7, 750 bp; gapdh, 1200 bp.

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