Fig. 5.

fabp7 mRNA is diurnally expressed in hypothalamus and forebrain. (A) Twenty-four hour profiles of the expression of fabp7 mRNA in VLPO, SCN and LH. In the upper panel, fabp7 mRNA levels are expressed relative to the average value of all samples normalized to gapdh. Each value represents the average±S.E.M. of RNA pooled from three individual animals that were independent from those used to probe DNA microarrays. SCN, N=3; VLPO, LH, N=2. SCN data were subjected to one-way ANOVA and a statistically significant circadian effect was revealed (P=0.002). Power of ANOVA with α=0.05: 0.952. ^* P<0.05, ^** P<0.01 vs. ZT16 (post hoc Bonferroni). The lower panel illustrates representative Northern blot analyses of fabp7 and gapdh in VLPO, SCN and LH. (B) In situ hybridization at the level of the dorsal hippocampus (H) taken at four time points confirmed diurnal changes in fabp7 mRNA expression. fabp7 mRNA was expressed primarily in cells surrounding the ventricles, although low levels of expression were evident throughout the forebrain. Peak levels of fapb7 mRNA expression occurred during the onset of the light period. T, thalamus.