Table 2.
Validated genes enriched in SCN, VLPO or LH
Gene | Region of enrichment | Unigene # | Fold enriched over other regions: microarray | Fold enriched over other regions: Northern blot | References that confirm enrichment |
---|---|---|---|---|---|
Smst | SCN | Mm.2453 | 3.5 | 2.2 | Tanaka et al., 1996 |
cited1 | SCN | Mm.42175 | 2.4 | 4.8 | First report |
Nurr1 | LH | Mm.3507 | 2.9 | 2.1 | Xiao et al., 1996 |
Ptx2 | LH | Mm.1385 | 44 | 3.5 | Smidt et al., 2000 |
CGA | VLPO | Mm.1361 | 11 | 14.7 | Schorr-Toshav et al., 1983 |
A number of genes enriched in SCN, VLPO or LH based on microarray analysis were validated using Northern blotting. Fold enrichment over an average value of the other two brain regions is indicated for microarray and Northern blots. Microarray data represent comparisons from four chips per region. Northern blots represent pooled RNA samples from 24 animals (see supplemental data). CGA, α-subunit of glycoprotein hormones; Smst, somatostatin.