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. Author manuscript; available in PMC: 2006 Oct 13.
Published in final edited form as: Transfusion. 2005 Sep;45(9):1487–1495. doi: 10.1111/j.1537-2995.2005.00561.x

Fig. 1.

Fig. 1

Detection of the HPA-9b (Maxa) mutation with real-time PCR. HPA-9a/b and HPA-9a/a DNA samples were amplified in the presence of SYBR Green with flanking primer Sex25 and the HPA-9b-specific primer (M) or the common allele-specific primer (W) curve fit relative fluorescence units (Table 1). Specific products are apparent after 21 cycles; primer-dimers are not apparent after 34 cycles.