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. Author manuscript; available in PMC: 2006 Oct 13.
Published in final edited form as: Transfusion. 2005 Sep;45(9):1487–1495. doi: 10.1111/j.1537-2995.2005.00561.x

TABLE 1.

Primers for HPA-9a/b (Maxa/b) amplification

Name Sequence PCR conditions
25S S* 5′-CCTCAGCATCCACCTTCCGGG-3′ 58°C 30 sec, 72°C 30 sec, 95°C 30 sec
26A A* 5′-GAGAACTGGATCCTGAAGCC-3′
n25S S 5′-GCCCTCCGACCTGCTCTACATC-3′ 66°C 45 sec, 72°C 30 sec, 95°C 30 sec
n25A A 5′-GCTCGGGCTCTGGCAGGAAG-3′
Sex25 S 5′-GCCTCCTGTCAACCCT-3′ 60°C 1 min, 95°C 25 sec
M A Maxa-specific 5′-GCAGCCCCCAGTCCAT-3′
W A wild-type-specific 5′-GCAGCCCCCAGTCCAC-3′
SMax S 5′-CTCTCAAGATGGACTGGG-3′ 50°C 30 sec, 72°C 1.5 min, 95°C 30 sec
AMax A 5′-CCCAGTCCATCTTGAGAG-3′ 50°C 30 sec, 72°C 30 sec, 95°C 30 sec
S1255 S 5′-CAAGTGCTGGTGTTCCTG-3′
A2865 A 5′-CAGCACAAACTGATCCAGAGGC-3′
*

S = sense; A = antisense.

Bold nucleotide is mutagenic.