TABLE 1.
Name | Sequence | PCR conditions |
---|---|---|
25S | S* 5′-CCTCAGCATCCACCTTCCGGG-3′ | 58°C 30 sec, 72°C 30 sec, 95°C 30 sec |
26A | A* 5′-GAGAACTGGATCCTGAAGCC-3′ | |
n25S | S 5′-GCCCTCCGACCTGCTCTACATC-3′ | 66°C 45 sec, 72°C 30 sec, 95°C 30 sec |
n25A | A 5′-GCTCGGGCTCTGGCAGGAAG-3′ | |
Sex25 | S 5′-GCCTCCTGTCAACCCT-3′ | 60°C 1 min, 95°C 25 sec |
M | A Maxa-specific 5′-GCAGCCCCCAGTCCAT-3′ | |
W | A wild-type-specific 5′-GCAGCCCCCAGTCCAC-3′ | |
SMax | S 5′-CTCTCAAGATGGACTGGG-3′† | 50°C 30 sec, 72°C 1.5 min, 95°C 30 sec |
AMax | A 5′-CCCAGTCCATCTTGAGAG-3′† | 50°C 30 sec, 72°C 30 sec, 95°C 30 sec |
S1255 | S 5′-CAAGTGCTGGTGTTCCTG-3′ | |
A2865 | A 5′-CAGCACAAACTGATCCAGAGGC-3′ |
S = sense; A = antisense.
Bold nucleotide is mutagenic.