Figure 1.

TMA design for melanoma progression. Cylinders corresponding to nevus (A), radial growth phase (B), vertical growth phase (C), and metastases (D). Immunohistochemical patterns. Core samples with positive and negative (or high and low) expression for selected antibodies are shown. All of the cases are representative of nevus-column A, and of each malignant melanoma progression step-columns B, C, and D. Note the high expression of cyclin D1 in radial (B) and metastases (D) when compared with vertical cases (C) and the loss of p16^INK4a expression in metastases. Note also the loss of p27^KIP1 and caveolin with tumor progression and the nuclear survivin expression in melanomas when compared with nevi. Original magnification for A–D, ×40; insets, ×600 and ×800, respectively. HE, hematoxylin and eosin.