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Western blot analysis of a dilution series of cerebral cortex (CC) and concentrated tonsil (To) extract from a case of vCJD (V7). The serial dilution was by a factor of two, starting with a loading of 5 μl of a 10% extract of frontal cortex in the first lane. The lane of tonsil represents 200 μl of a 10% extract enriched (×40) by centrifugal concentration for PrPres.
