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. 2004 Jan;164(1):273–283. doi: 10.1016/S0002-9440(10)63117-3

Figure 2.

Figure 2

Characterization of stable TSU-Pr1 transfectants. A: Immunoblot analysis with anti-pp32 antibody. Lanes A and B, AS-32; lane C, vector control. The Ponceau S-stained membrane shown below the blot demonstrates that equal amounts of protein were loaded onto each lane. Lanes A and B represent two different lines of the AS-32 cells. B: Quantitative analysis of pp32-specific mRNA by competitive RT-PCR. The graph shows the actual copy numbers computed from densitometric calibration curves as described in Material and Methods. C: The transfected anti-sense construct is expressed by RT-PCR analysis. AS-32 cells stably transfected with pp32 anti-sense express the anti-sense pp32 construct mRNA whereas vector control transfectants do not express the anti-sense strand. D: pp32 RNAi abolishes expression of pp32. Top: A Western blot demonstrating that no pp32 is detected in TSU-Pr1 cells treated with pp32 RNAi, whereas pp32 continues to be expressed in cells treated with the scrambled control RNAi construct. Bottom: A Ponceau S stain of the membrane, demonstrating loading of equal amounts of protein.