Figure 5.

Analysis of histone acetylation. A: Immunoblot analysis of histone acetylation. AS-32 cells stably transfected with pp32 anti-sense showed a reduced expression of hyperacetylated histone H4 compared to cells transfected with vector only. B: Effect of pp32 RNAi on histone acetylation. The figure shows Western blots demonstrating that pp32 RNAi induces decreased levels of acetylated histone H4 in treated TSU-Pr1 cells as compared to the scrambled control. Only acetylation was affected because analysis of total histone H4 protein demonstrated comparable levels in both control and RNAi-treated cells, and the Ponceau S stain confirmed that equal amounts of protein were loaded.