Table 1.
Antibody | Source | Dilution | Antigen retrieval*
|
|||
---|---|---|---|---|---|---|
Buffer | pH | Time | Temperature | |||
Survivin | Novus | 1:1000 | Cytrate | 6 | 5 min | 100°C |
Cleaved caspase-3 | Cell Signaling | 1:10 | EDTA | 8 | 2 min | 100°C |
Ki-67 (MIB-1) | Immunotech | 1:400 | Cytrate | 6 | 5 min | 100°C |