Skip to main content
. 2004 Feb;164(2):409–417. doi: 10.1016/S0002-9440(10)63131-8

Figure 2.

Figure 2

The Th1-producing T cells lose the expression of CD3 but not that of CD4 after oligoclonal and polyclonal activation. Double staining using anti-IL-17 or anti-IFN-γ (brown) and anti-CD4 or anti-CD3 antibodies (blue) were performed in the two in vitro models. After 4 hours of stimulation with PMA/PHA, IFN-γ+ cells express the CD3 marker (A and B). After 24 hours, the lack of CD3 expression on IFN-γ-producing cells (C) was also observed after 48 hours (D and E). Double staining using anti-IL-17 (brown) and anti-CD4 (blue) shows that IL-17-producing cells express the CD4 after 4, 24, and 48 hours (F, G, H, and I, respectively). After 4 hours of stimulation with the superantigen SEB, the expression of CD3 is still present on IFN-γ+CD4+ cell subset (J) but with a lower staining intensity when compared to that of CD3 staining on IFN-γ−CD4+ cell subset (K). Original magnifications, ×1000.