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. 2004 Feb;164(2):409–417. doi: 10.1016/S0002-9440(10)63131-8

Figure 3.

Figure 3

Kinetic of CD3 and IFN-γ expression on CD4+ T cells after oligoclonal activation with SEB. Using a FACS analysis, the CD3 kinetics (detected with anti-CD3-fluorescein isothiocyanate) were evaluated from 0 to 6 hours on SEB stimulation on CD4 T cells in combination with IFN-γ staining (detected with anti-IFN-γ-PE). The dot-plots display CD3 versus IFN-γ staining gated on CD4+ cells isolated from PBMCs stimulated from 0 to 6 hours. The loss of CD3 expression on CD4+ T cells is time-dependent and associated with that of IFN-γ apparition.