Table 1.
Estimation of Single-Cell Production of IFN-γ and IL-17
| Duration of cultures
|
|||
|---|---|---|---|
| 4 hours | 24 hours | 48 hours | |
| Concentrations of cytokines in PBMC supernatants (1 × 106 cells/ml) (ng/ml) | |||
| IFN-γ | 24.3 ± 5.9 | 70 ± 8.2 | 168 ± 11.4 |
| IL-17 | 7.4 ± 1.9 | 9.8 ± 0.7 | 17.8 ± 0.3 |
| Number of cytokine-producing cells on cytospin slides (n = 5) (2.105 cells per slide) | |||
| IFN-γ | 4103 ± 402 | 10217 ± 421 | 11100 ± 350 |
| IL-17 | 1108 ± 116 | 1200 ± 190 | 2205 ± 180 |
| Single-cell production of cytokines (pg) | |||
| IFN-γ | 1.1 | 1.4 | 3 |
| IL-17 | 1.3 | 1.6 | 2.5 |
PBMCs were stimulated for 4, 24, or 48 hours with PMA (5 ng/ml) and PHA (1 μg/ml). Cytokine levels were measured in the supernatants by ELISA. The number of IL-17+ and IFN-γ+ cells on cytospin slides was evaluated using the Lucia image analyzer.