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. 2004 Feb;164(2):679–688. doi: 10.1016/S0002-9440(10)63155-0

Figure 8.

Figure 8

Differential effect of estrogen on VEGF expression in renal tubular cells and VSMCs. 17βE induced an up-regulation of VEGF mRNA and protein both in NRK52E cells and VSMCs assessed by RNase protection assay (A, n = 6) and enzyme-linked immunosorbent assay (B, n = 4), respectively. However, pretreatment with a NO donor resulted in attenuation of 17βE-induced VEGF expression only in VSMCs. A: Shown is a representative RNase protection assay blot. E, 17βE; SNP, sodium nitroprusside; SIN, 3-morpholinosydnonomine. *, P < 0.05 versus control; #, P < 0.05 versus control, E+SNP, and E+SIN. Data are expressed as mean ± SD.