Figure 11.

Co-localization of smooth muscle myosin and caldesmon in bladder myocytes from decompensated bladder. A: Immunostaining of myocytes dissociated from bladder wall smooth muscle reacted with FITC-conjugated antibody specific to caldesmon. B: Immunostaining of myocytes dissociated from bladder wall smooth muscle reacted with Texas Red-conjugated antibody specific to smooth muscle myosin. C: Combined fluorescence showing co-localization of myosin and caldesmon antibodies in bladder myocytes and cytoplasmic fibrils D: Photographs of the cells shown in A, B, and C taken under differential interference contrast. Notice that the cytoplasmic fibrils in the myocytes react with smooth muscle-specific myosin and caldesmon. There is a large amount of immunostaining with caldesmon antibody in the area around the nuclei.