Figure 12.

Specific immunostaining of h-CaD in bladder myocytes dissociated from decompensated bladder. A: Myocytes were treated with unlabeled antibody against the C-terminal region of CaD before immunoreaction with Texas red labeled h-CaD-specific antibody. B: Bladder myocytes in the same field as A, shown under differential interference contrast. Notice that the majority of the cells dissociated from the detrusor smooth muscle from decompensated bladder stains positive for h-CaD, which is smooth muscle-specific, indicating the differentiated state of the myocytes in the detrusor.