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. 2004 Feb;164(2):689–699. doi: 10.1016/S0002-9440(10)63156-2

Figure 3.

Figure 3

Examples of PCR products from DNA methylation analyses of multiple CpG islands in patients with gastric cancer (S). N, noncancerous mucosae; T, cancer tissue. The DNA methylation status of the CpG islands of the p16 (A), hMLH1 (B), and E-cadherin (C) genes was evaluated by MSP. In this analysis, the PCR products generated by primer sets M and U reflect the presence of methylated and unmethylated genes, respectively. Primer set W was used to confirm the completeness of the bisulfite modification. A: Methylated gene was detected in S24N, but the signal intensity of the M fragment was markedly increased in S24T compared with S24N. This indicates that a greater number of cells had undergone DNA hypermethylation in T than in N, although N can contain precursor cells for cancers and/or precancerous lesions. DNA methylation of the CpG islands of the THBS-1 (D) gene and the MINT-1, -2, -12, and -31 clones (E, F, G, and H, respectively) was evaluated by COBRA. In this analysis, only methylated genes (arrows) were digested by the restriction enzymes.