Figure 4.

The JCV receptor-type SA but not the nonreceptor-type SA is present on B lymphocytes in the spleen. Spleen sections were double-labeled with monoclonal anti-CD19 antibody and biotinylated SNA (A) or MAA (B) lectin overnight at 4°C. CD19 binding was visualized with Texas Red-conjugated goat anti-mouse Ab, and lectin binding was detected with fluorescein dichlorotriazine-conjugated streptavidin. Both panels in A demonstrate co-localization in the binding reactivity of anti-CD19 Ab and the SNA lectin, whereas no co-staining is seen with the MAA lectin in CD-19-positive regions in the spleen.